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ace2 sars cov 2 spike s1 inhibitor screening assay kit  (BPS Bioscience)


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    BPS Bioscience ace2 sars cov 2 spike s1 inhibitor screening assay kit
    Ace2 Sars Cov 2 Spike S1 Inhibitor Screening Assay Kit, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sars+cov+2+spike+ace2+inhibitor+screening+assay+kit/Spike+S1+(SARS-CoV-2)%3A+ACE2+Inhibitor+Screening+Colorimetric+Assay+Kit/pm41342557-124-15-22
    Average 94 stars, based on 19 article reviews
    ace2 sars cov 2 spike s1 inhibitor screening assay kit - by Bioz Stars, 2026-10
    94/100 stars

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    Screening Assay:

    Article Title: Mulberry Component Kuwanon C Exerts Potent Therapeutic Efficacy In Vitro against COVID-19 by Blocking the SARS-CoV-2 Spike S1 RBD:ACE2 Receptor Interaction.
    Article Snippet: KC was purchased from ChemFaces (Wuhan, China). .. The SARS-CoV-2 spike/ACE2 inhibitor screening assay kit, biotin-labeled recombinant protein ACE2 receptor, and spike protein S1 RBD (BPS Bioscience, San Diego, CA, USA) were purchased for the competitive ELISA assay and BLItz analysis. ..

    Article Title: Mulberry Component Kuwanon C Exerts Potent Therapeutic Efficacy In Vitro against COVID-19 by Blocking the SARS-CoV-2 Spike S1 RBD:ACE2 Receptor Interaction
    Article Snippet: KC was purchased from ChemFaces (Wuhan, China). .. The SARS-CoV-2 spike/ACE2 inhibitor screening assay kit, biotin-labeled recombinant protein ACE2 receptor, and spike protein S1 RBD (BPS Bioscience, San Diego, CA, USA) were purchased for the competitive ELISA assay and BLItz analysis. .. Human ACE2/TMPRSS2-overexpressing HEK293T stable cell line was purchased from GeneCopoeia (Rockville, MD, USA) and maintained in Dulbecco’s modified Eagle’s medium (Lonza, Walkersville, MD, USA) containing 10% fetal bovine serum (Biotechnics Research, Lake Forest, CA, USA) and 1% penicillin/streptomycin (Cellgro, Manassas, VA, USA) at 37 °C in a 5% CO 2 incubator.

    Article Title: Mulberry Component Kuwanon C Exerts Potent Therapeutic Efficacy In Vitro against COVID-19 by Blocking the SARS-CoV-2 Spike S1 RBD:ACE2 Receptor Interaction
    Article Snippet: .. The binding of KC to the SARS-CoV-2 spike protein and the ACE2 receptor was analyzed using the SARS-CoV-2 spike/ACE2 inhibitor screening assay kit (BPS Bioscience, San Diego, CA, USA). ..

    Article Title: Mulberrofuran G, a Mulberry Component, Prevents SARS-CoV-2 Infection by Blocking the Interaction between SARS-CoV-2 Spike Protein S1 Receptor-Binding Domain and Human Angiotensin-Converting Enzyme 2 Receptor
    Article Snippet: .. The SARS-CoV-2 spike/ACE2 inhibitor screening assay kit, biotin-labeled recombinant protein ACE2 receptor, and spike protein S1 RBD (BPS Bioscience, San Diego, CA, USA) were procured for biolayer interferometry. .. HEK293T cells stably expressing human ACE2 and TMPRSS2 cells were obtained from GeneCopoeia (Rockville, MD, USA) and maintained in Dulbecco’s modified Eagle’s medium (Lonza, Walkersville, MD, USA) containing 10% fetal bovine serum (Biotechnics Research, Lake Forest, CA, USA) and 1% penicillin/streptomycin (Cellgro, Manassas, VA, USA) at 37 °C in a 5% CO 2 incubator.

    Article Title: The SpACE-CCM: A facile and versatile cell culture medium-based biosensor for detection of SARS-CoV-2 spike-ACE2 interaction.
    Article Snippet: .. Sera containing neutralizing antibodies and small molecules that inhibited the spike RBD-ACE2 interaction were also analyzed using a SARS-CoV-2 Spike:ACE2 Inhibitor Screening Assay Kit (BPS Bioscience, CA, USA, Cat.# 79931), according to the manufacturer’s instructions. ..

    Article Title: Mulberry Component Kuwanon C Exerts Potent Therapeutic Efficacy In Vitro against COVID-19 by Blocking the SARS-CoV-2 Spike S1 RBD:ACE2 Receptor Interaction.
    Article Snippet: .. The binding of KC to the SARS-CoV-2 spike protein and the ACE2 receptor was analyzed using the SARS-CoV-2 spike/ACE2 inhibitor screening assay kit (BPS Bioscience, San Diego, CA, USA). ..

    Article Title: The SpACE-CCM: A facile and versatile cell culture medium-based biosensor for detection of SARS-CoV-2 spike-ACE2 interaction
    Article Snippet: .. Sera containing neutralizing antibodies and small molecules that inhibited the spike RBD-ACE2 interaction were also analyzed using a SARS-CoV-2 Spike:ACE2 Inhibitor Screening Assay Kit (BPS Bioscience, CA, USA, Cat.# 79931), according to the manufacturer’s instructions. ..

    Article Title: Phenothiazines inhibit SARS-CoV-2 cell entry via a blockade of spike protein binding to neuropilin-1
    Article Snippet: Cell surface expression of ACE2 was analyzed by flow cytometry using SH800S (SONY, Tokyo, Japan) and data were analyzed with Cell Sorter Software (SONY). .. The effects of the test compounds and the neutralizing antibody R001 on SARS2-S binding to ACE2 were analyzed with a SARS-CoV-2 Spike:ACE2 Inhibitor Screening Assay Kit (BPS Bioscience, San Diego, CA, USA). ..

    Recombinant:

    Article Title: Mulberry Component Kuwanon C Exerts Potent Therapeutic Efficacy In Vitro against COVID-19 by Blocking the SARS-CoV-2 Spike S1 RBD:ACE2 Receptor Interaction.
    Article Snippet: KC was purchased from ChemFaces (Wuhan, China). .. The SARS-CoV-2 spike/ACE2 inhibitor screening assay kit, biotin-labeled recombinant protein ACE2 receptor, and spike protein S1 RBD (BPS Bioscience, San Diego, CA, USA) were purchased for the competitive ELISA assay and BLItz analysis. ..

    Article Title: Mulberry Component Kuwanon C Exerts Potent Therapeutic Efficacy In Vitro against COVID-19 by Blocking the SARS-CoV-2 Spike S1 RBD:ACE2 Receptor Interaction
    Article Snippet: KC was purchased from ChemFaces (Wuhan, China). .. The SARS-CoV-2 spike/ACE2 inhibitor screening assay kit, biotin-labeled recombinant protein ACE2 receptor, and spike protein S1 RBD (BPS Bioscience, San Diego, CA, USA) were purchased for the competitive ELISA assay and BLItz analysis. .. Human ACE2/TMPRSS2-overexpressing HEK293T stable cell line was purchased from GeneCopoeia (Rockville, MD, USA) and maintained in Dulbecco’s modified Eagle’s medium (Lonza, Walkersville, MD, USA) containing 10% fetal bovine serum (Biotechnics Research, Lake Forest, CA, USA) and 1% penicillin/streptomycin (Cellgro, Manassas, VA, USA) at 37 °C in a 5% CO 2 incubator.

    Article Title: Mulberrofuran G, a Mulberry Component, Prevents SARS-CoV-2 Infection by Blocking the Interaction between SARS-CoV-2 Spike Protein S1 Receptor-Binding Domain and Human Angiotensin-Converting Enzyme 2 Receptor
    Article Snippet: .. The SARS-CoV-2 spike/ACE2 inhibitor screening assay kit, biotin-labeled recombinant protein ACE2 receptor, and spike protein S1 RBD (BPS Bioscience, San Diego, CA, USA) were procured for biolayer interferometry. .. HEK293T cells stably expressing human ACE2 and TMPRSS2 cells were obtained from GeneCopoeia (Rockville, MD, USA) and maintained in Dulbecco’s modified Eagle’s medium (Lonza, Walkersville, MD, USA) containing 10% fetal bovine serum (Biotechnics Research, Lake Forest, CA, USA) and 1% penicillin/streptomycin (Cellgro, Manassas, VA, USA) at 37 °C in a 5% CO 2 incubator.

    Competitive ELISA:

    Article Title: Mulberry Component Kuwanon C Exerts Potent Therapeutic Efficacy In Vitro against COVID-19 by Blocking the SARS-CoV-2 Spike S1 RBD:ACE2 Receptor Interaction.
    Article Snippet: KC was purchased from ChemFaces (Wuhan, China). .. The SARS-CoV-2 spike/ACE2 inhibitor screening assay kit, biotin-labeled recombinant protein ACE2 receptor, and spike protein S1 RBD (BPS Bioscience, San Diego, CA, USA) were purchased for the competitive ELISA assay and BLItz analysis. ..

    Article Title: Mulberry Component Kuwanon C Exerts Potent Therapeutic Efficacy In Vitro against COVID-19 by Blocking the SARS-CoV-2 Spike S1 RBD:ACE2 Receptor Interaction
    Article Snippet: KC was purchased from ChemFaces (Wuhan, China). .. The SARS-CoV-2 spike/ACE2 inhibitor screening assay kit, biotin-labeled recombinant protein ACE2 receptor, and spike protein S1 RBD (BPS Bioscience, San Diego, CA, USA) were purchased for the competitive ELISA assay and BLItz analysis. .. Human ACE2/TMPRSS2-overexpressing HEK293T stable cell line was purchased from GeneCopoeia (Rockville, MD, USA) and maintained in Dulbecco’s modified Eagle’s medium (Lonza, Walkersville, MD, USA) containing 10% fetal bovine serum (Biotechnics Research, Lake Forest, CA, USA) and 1% penicillin/streptomycin (Cellgro, Manassas, VA, USA) at 37 °C in a 5% CO 2 incubator.

    Binding Assay:

    Article Title: Mulberry Component Kuwanon C Exerts Potent Therapeutic Efficacy In Vitro against COVID-19 by Blocking the SARS-CoV-2 Spike S1 RBD:ACE2 Receptor Interaction
    Article Snippet: .. The binding of KC to the SARS-CoV-2 spike protein and the ACE2 receptor was analyzed using the SARS-CoV-2 spike/ACE2 inhibitor screening assay kit (BPS Bioscience, San Diego, CA, USA). ..

    Article Title: Mulberry Component Kuwanon C Exerts Potent Therapeutic Efficacy In Vitro against COVID-19 by Blocking the SARS-CoV-2 Spike S1 RBD:ACE2 Receptor Interaction.
    Article Snippet: .. The binding of KC to the SARS-CoV-2 spike protein and the ACE2 receptor was analyzed using the SARS-CoV-2 spike/ACE2 inhibitor screening assay kit (BPS Bioscience, San Diego, CA, USA). ..

    Article Title: Phenothiazines inhibit SARS-CoV-2 cell entry via a blockade of spike protein binding to neuropilin-1
    Article Snippet: Cell surface expression of ACE2 was analyzed by flow cytometry using SH800S (SONY, Tokyo, Japan) and data were analyzed with Cell Sorter Software (SONY). .. The effects of the test compounds and the neutralizing antibody R001 on SARS2-S binding to ACE2 were analyzed with a SARS-CoV-2 Spike:ACE2 Inhibitor Screening Assay Kit (BPS Bioscience, San Diego, CA, USA). ..



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    <t>RBD-ACE2</t> interaction <t>inhibition</t> assays. ( a ) Schematic illustration of ELISA experiment to evaluate the ability of the A13 or Nat1 to prevent the interaction of SARS-CoV-2 S1 protein RBD to human receptor ACE2. First the plates were incubated with immobilized RBD. They were then inoculated with peptides prior to the addition of recombinant human receptor ACE2. ( b ) Inhibition of RBD-ACE2 binding via A13 or Nat1. The level of RBD-ACE2 binding was measured by chemiluminescence. The negative control with no peptide representing RBD-ACE2 interaction was set to 1.00 (3.2 × 10 4 RLU) and other values were normalized to this number. To the same extent, at the concentration of 0.1 µg/mL, both peptides reduced ACE2 binding compared to the control. At 1.0 µg/mL, inhibition was further enhanced. The negative control peptide did not exhibit a notable inhibition. Data represent the mean value from at least three independent experiments. Error bars represent standard deviation. Statistical significance was determined using the nonparametric two-tailed Student’s t -test (**** p ≤ 0.0001).
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    Image Search Results


    RBD-ACE2 interaction inhibition assays. ( a ) Schematic illustration of ELISA experiment to evaluate the ability of the A13 or Nat1 to prevent the interaction of SARS-CoV-2 S1 protein RBD to human receptor ACE2. First the plates were incubated with immobilized RBD. They were then inoculated with peptides prior to the addition of recombinant human receptor ACE2. ( b ) Inhibition of RBD-ACE2 binding via A13 or Nat1. The level of RBD-ACE2 binding was measured by chemiluminescence. The negative control with no peptide representing RBD-ACE2 interaction was set to 1.00 (3.2 × 10 4 RLU) and other values were normalized to this number. To the same extent, at the concentration of 0.1 µg/mL, both peptides reduced ACE2 binding compared to the control. At 1.0 µg/mL, inhibition was further enhanced. The negative control peptide did not exhibit a notable inhibition. Data represent the mean value from at least three independent experiments. Error bars represent standard deviation. Statistical significance was determined using the nonparametric two-tailed Student’s t -test (**** p ≤ 0.0001).

    Journal: International Journal of Molecular Sciences

    Article Title: Artificial Intelligence Reveals Nature: Functional Parallels Between a Designed and a Natural Peptide

    doi: 10.3390/ijms262110607

    Figure Lengend Snippet: RBD-ACE2 interaction inhibition assays. ( a ) Schematic illustration of ELISA experiment to evaluate the ability of the A13 or Nat1 to prevent the interaction of SARS-CoV-2 S1 protein RBD to human receptor ACE2. First the plates were incubated with immobilized RBD. They were then inoculated with peptides prior to the addition of recombinant human receptor ACE2. ( b ) Inhibition of RBD-ACE2 binding via A13 or Nat1. The level of RBD-ACE2 binding was measured by chemiluminescence. The negative control with no peptide representing RBD-ACE2 interaction was set to 1.00 (3.2 × 10 4 RLU) and other values were normalized to this number. To the same extent, at the concentration of 0.1 µg/mL, both peptides reduced ACE2 binding compared to the control. At 1.0 µg/mL, inhibition was further enhanced. The negative control peptide did not exhibit a notable inhibition. Data represent the mean value from at least three independent experiments. Error bars represent standard deviation. Statistical significance was determined using the nonparametric two-tailed Student’s t -test (**** p ≤ 0.0001).

    Article Snippet: The ability of the peptides to block the interaction between the RBD and ACE2 was assessed using a commercial ACE2 inhibition assay kit (BPS Bioscience, San Diego, CA, United States, CAT# 79931).

    Techniques: Inhibition, Enzyme-linked Immunosorbent Assay, Incubation, Recombinant, Binding Assay, Negative Control, Concentration Assay, Control, Standard Deviation, Two Tailed Test

    NanoLuc bioreporter assays. ( a ) A schematic illustration for the NanoLuc bioreporter assay to determine SARS-CoV-2 S1 RBD—ACE2 inhibition by A13 or Nat1. ( b ) NanoLuc bioreporter assay indicates that both peptides reduce luciferase complementation to the same extent. The signal value for no peptide control was set to 1.00 (6.30 × 10 5 RLU) and all other values were normalized to it. In cell lysates, A13 and Nat1 both reduced luminescence, indicating inhibition of RBD-ACE2 binding. The negative control peptide had no significant effect. Data represent the mean value from at least three independent experiments. Error bars represent standard deviation. Statistical significance was determined using the nonparametric two-tailed Student’s t -test (**** p ≤ 0.0001, ** p ≤ 0.01).

    Journal: International Journal of Molecular Sciences

    Article Title: Artificial Intelligence Reveals Nature: Functional Parallels Between a Designed and a Natural Peptide

    doi: 10.3390/ijms262110607

    Figure Lengend Snippet: NanoLuc bioreporter assays. ( a ) A schematic illustration for the NanoLuc bioreporter assay to determine SARS-CoV-2 S1 RBD—ACE2 inhibition by A13 or Nat1. ( b ) NanoLuc bioreporter assay indicates that both peptides reduce luciferase complementation to the same extent. The signal value for no peptide control was set to 1.00 (6.30 × 10 5 RLU) and all other values were normalized to it. In cell lysates, A13 and Nat1 both reduced luminescence, indicating inhibition of RBD-ACE2 binding. The negative control peptide had no significant effect. Data represent the mean value from at least three independent experiments. Error bars represent standard deviation. Statistical significance was determined using the nonparametric two-tailed Student’s t -test (**** p ≤ 0.0001, ** p ≤ 0.01).

    Article Snippet: The ability of the peptides to block the interaction between the RBD and ACE2 was assessed using a commercial ACE2 inhibition assay kit (BPS Bioscience, San Diego, CA, United States, CAT# 79931).

    Techniques: Inhibition, Luciferase, Control, Binding Assay, Negative Control, Standard Deviation, Two Tailed Test

    Pseudovirus infectivity assays. ( a ) A schematic illustration for pseudovirus infectivity assay to determine the inhibition ability of the peptides to prevent psuedovirus to enter the cell. ( b ) Pseudovirus infectivity assays indicates that both peptides exhibit similar effects on pseudovirus infected cells. The signal value for pseudovirus infection with no peptide was set to 1.00 and corresponding values were normalized to it. Cells were incubated with pseudovirus in the absence/presence of A13 or Nat1, and infection was measured using a luciferase reporter. Both peptides reduce pseudovirus entry into ACE2-expressing cells. Neither peptide shows any visible effects on the non-peptide sample. Data represents the mean value from at least three independent experiments. Error bars represent standard deviation. Statistical significance was determined using the nonparametric two-tailed Student’s t -test (**** p ≤ 0.0001, ** p ≤ 0.01).

    Journal: International Journal of Molecular Sciences

    Article Title: Artificial Intelligence Reveals Nature: Functional Parallels Between a Designed and a Natural Peptide

    doi: 10.3390/ijms262110607

    Figure Lengend Snippet: Pseudovirus infectivity assays. ( a ) A schematic illustration for pseudovirus infectivity assay to determine the inhibition ability of the peptides to prevent psuedovirus to enter the cell. ( b ) Pseudovirus infectivity assays indicates that both peptides exhibit similar effects on pseudovirus infected cells. The signal value for pseudovirus infection with no peptide was set to 1.00 and corresponding values were normalized to it. Cells were incubated with pseudovirus in the absence/presence of A13 or Nat1, and infection was measured using a luciferase reporter. Both peptides reduce pseudovirus entry into ACE2-expressing cells. Neither peptide shows any visible effects on the non-peptide sample. Data represents the mean value from at least three independent experiments. Error bars represent standard deviation. Statistical significance was determined using the nonparametric two-tailed Student’s t -test (**** p ≤ 0.0001, ** p ≤ 0.01).

    Article Snippet: The ability of the peptides to block the interaction between the RBD and ACE2 was assessed using a commercial ACE2 inhibition assay kit (BPS Bioscience, San Diego, CA, United States, CAT# 79931).

    Techniques: Infection, Inhibition, Incubation, Luciferase, Expressing, Standard Deviation, Two Tailed Test